ACCURATE DETECTION AND QUANTIFICATION OF SEASONAL ABUNDANCE OF AMERICAN BULLFROG (LITHOBATES CATESBEIANUS) USING DDPCR EDNA ASSAYS

Accurate detection and quantification of seasonal abundance of American bullfrog (Lithobates catesbeianus) using ddPCR eDNA assays

Abstract The invasive American bullfrog (Lithobates catesbeianus) imperils freshwater biodiversity worldwide.Effective management hinges on early detection of incipient invasions and subsequent rapid response, as established populations are extremely difficult to eradicate.Although environmental DNA (eDNA) detection methods provide a highly sensiti

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Targeted modulation of gut microbiota by traditional Chinese medicine and natural products for liver disease therapy

The gut microbiota not only constitutes intestinal microenvironment homeostasis and human health but also exerts indispensable roles in the occurrence and progression of multiple liver diseases, including alcohol-related liver disease, nonalcoholic fatty liver disease, autoimmune liver Hand Strap disease and liver cancer.Given the therapeutic statu

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Pain and Problem Behavior in Cats and Dogs

We argue that there is currently an under-reporting of the ways in which pain can be associated with problem behavior, which is seriously limiting the recognition of this welfare problem.A review of the caseloads of 100 recent dog cases of several authors indicates that a conservative estimate of around a third of referred cases involve some form o

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