Accurate detection and quantification of seasonal abundance of American bullfrog (Lithobates catesbeianus) using ddPCR eDNA assays
Abstract The invasive American bullfrog (Lithobates catesbeianus) imperils freshwater biodiversity worldwide.Effective management hinges on early detection of incipient invasions and subsequent rapid response, as established populations are extremely difficult to eradicate.Although environmental DNA (eDNA) detection methods provide a highly sensiti